Tunel 检测
TUNEL（TdT-mediated dUTP nick end labeling）检测是用来检测组织细胞在凋亡早期过程中细胞核DNA的断裂情况，原理是生物素biotinylate标记的dUTP在脱氧核糖核苷酸末端转移酶（TdT Enzyme）的作用下，可以连接到凋亡细胞中断裂DNA的3’-OH末端，并与连接辣根过氧化酶（HRP，horse-radish peroxidase）的链霉亲和素streptavidin特异性结合，后者又与POD底物H2O2、二氨基联苯胺（DAB）产生深棕色反应，特异准确地定位正在凋亡的细胞，因而在光学显微镜下即可观察凋亡细胞；由于正常的或正在增殖的细胞几乎没有DNA断裂，因而没有3‘-OH形成，很少能够被染色。
实验流程图
结果展示
| contact us Address: Room 402, West Block, Ganghongji Business Building, Nanshan District, Shenzhen 1107, Building 1, Hengtaiyu Building, Tongtong Road, Fenghuang Street, Guangming District, Shenzhen 11th Floor, Second Training Building, Guangdong Light Industry Vocational and Technical College, No. 152 Xingang West Road, Haizhu District, Guangzhou City Tel：0755-86325431 facsimile：0755-86325431 Service email：sales@dhfengyu.com |
