服务介绍
细胞克隆形成率即细胞接种存活率,表示接种细胞后贴壁的细胞成活并形成克隆的数量。贴壁后的细胞不一定每个都能增殖和形成克隆,而形成克隆的细胞必为贴壁和有增殖活力的细胞。克隆形成率能够反应细胞群体依赖性和增殖能力两个重要性状。
服务内容
1) 取对数生长期的细胞,分别用0.25%胰酶消化并吹打成单细胞状态;
2) 将细胞悬液作梯度倍数稀释,每组细胞分别以每皿50、100、200个细胞的梯度密度分别接种含培养基的培养皿中,轻轻转动,使细胞分散均匀。将培养皿置于37℃、5% CO2培养箱中培养2~3周(根据细胞种类和生长状况);
3) 经常观察,当培养皿中出现肉眼可见的克隆时,终止培养。弃去上清液,用PBS小心浸洗2次。加4%多聚甲醛固定
| contact us Address: Room 402, West Block, Ganghongji Business Building, Nanshan District, Shenzhen 1107, Building 1, Hengtaiyu Building, Tongtong Road, Fenghuang Street, Guangming District, Shenzhen 11th Floor, Second Training Building, Guangdong Light Industry Vocational and Technical College, No. 152 Xingang West Road, Haizhu District, Guangzhou City Tel:0755-86325431 facsimile:0755-86325431 Service email:sales@dhfengyu.com |